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KMID : 0364820140500020108
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Korean Journal of Microbiology
2014 Volume.50 No. 2 p.108 ~ p.113
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Time-course Analysis of Biofilm Formation in Quorum Sensing-deficient Bacteria
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Kim Su-kyoung
Lee Mi-Nan
Lee Joon-Hee
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Abstract
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Pseudomonas aeruginosa and Vibrio vulnificus are Gram-negative human pathogens, which exert their virulence
through quorum sensing (QS) regulation. The infection of these pathogens have been known to be mediated by
biofilm formation in many cases and this study carried out the time-course analysis of biofilm formation depending
on the QS regulation in P. aeruginosa and V. vulnificus. In P. aeruginosa, our results demonstrated that QS-deficient
mutant better attached to surface at initial stage of biofilm formation, but poorly proceeded to the maturation of the
biofilm structure, while wild type less attached at initial stage but developed highly structured biofilm at late stage.
Because of this, the quantitative comparison of biofilm formation between wild type and the QS mutant showed the
reversion; the QS mutant formed more biofilm until 10 h after inoculation than wild type, but wild type formed
much more biofilm after 10 h than QS mutant. V. vulnificus has been reported to form more biofilm with the
mutation on QS system. When we performed the same time-course analysis of the V. vulnificus biofilm formation,
the reversion was not detected even with prolonged culture for 108 h and the QS mutant always forms more biofilm
than wild type. These results indicate that the QS regulation negatively affects the attachment at early stage but
positively facilitates the biofilm maturation at late stage in P. aeruginosa, while the QS regulation has a negative
effect on the biofilm formation throughout the biofilm development in V. vulnificus. Based on our results, we suggest
that the developmental stage of biofilm and bacterial species should be considered when the QS system is targeted
for biofilm control.
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KEYWORD
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Pseudomonas aeruginosa, Vibrio vulnificus, biofilm formation, quorum sensing, time-course analysis
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